cortical3.mp4

The video shows cortical activity recorded through widefield calcium imaging. This video corresponds to Fig. 2.b in the manuscript (labeled as Visualization 1)

Media 1: A hematoma detector—a practical application of instrumental motion as signal in near infra-red imaging

Originally published in Biomedical Optics Express on 01 January 2012 (boe-3-1-192

Multi-spectral instrument.

<p>Linearly polarized light is projected onto the skin and diffuse reflectance images are captured at the CCD camera after passing narrow band filters for wavelength selection.</p

Eigenvector converted blood volume (EV1) and oxygenation (EV2) images.

<p>Two adjacent KS lesions over time are shown in the images in arbitrary units of a patient with a confirmed pathological complete response. A digital photograph of the lesions is shown at baseline (week 0).</p

Cross-sections through blood volume and blood oxygenation at baseline in a KS lesion.

<p>Cross-sections were chosen to be centered on the lesion, for both blood volume (a) and oxygenation (c). The EV1 and EV2 are depicted in arbitrary units as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0083887#pone-0083887-g002" target="_blank">Fig. 2</a>. The average of 5 cross sections for blood volume (b) shows the typical increase and for oxygenation (d) shows a decrease inside the lesion area. A surrounding halo of increased oxygenation can be seen, especially to the immediate right of the lesion. The width of the lines in (b) and (d) reflect the range of +/− 1 standard deviation for the 11 cross sections at each point.</p

Patient Characteristics and Response to Treatment.

<p>In patient 6, the target lesion flattened, but some other flat lesions became nodular and new lesions appeared, so the patient met the overall criteria for progressive disease.</p

Time courses of NESD for EV1 and EV2.

<p>(a) Time course for the NESD of blood volume (EV1) for 5 lesions that showed a clinical response (black) and two lesions that did not show a clinical response (grey). Patients whose lesion responded to treatment show a clear decrease in NESD of blood volume (EV1) over time, whereas those whose lesion progressed show an increase. (b) Time course for the NESD of blood oxygenation (EV2); again the 5 lesions that responded clinically are shown in black, and the 2 lesions that did not respond are shown in grey. The errobars shown correspond to the error on the estimate of the standard deviation. If the errorbar is not visible, the error is smaller than the marker size.</p

Eigenvector converted blood volume (EV1) and oxygenation (EV2) images.

<p>The KS lesion is shown over time in arbitrary units of a patient with progressive disease. A digital photograph of the lesion is shown at baseline (week 0).</p